diff --git a/stdlib/2/__builtin__.pyi b/stdlib/2/__builtin__.pyi
index a79300956..7a7245396 100644
--- a/stdlib/2/__builtin__.pyi
+++ b/stdlib/2/__builtin__.pyi
@@ -56,6 +56,7 @@ from typing import (
     Union,
     ValuesView,
     overload,
+    runtime_checkable,
 )
 from typing_extensions import Literal
 
@@ -1211,7 +1212,7 @@ if sys.version_info < (3,):
 # but we define it here as _PathLike to avoid import cycle issues.
 # See https://github.com/python/typeshed/pull/991#issuecomment-288160993
 _AnyStr_co = TypeVar("_AnyStr_co", str, bytes, covariant=True)
-
+@runtime_checkable
 class _PathLike(Protocol[_AnyStr_co]):
     def __fspath__(self) -> _AnyStr_co: ...
 
diff --git a/stdlib/2and3/builtins.pyi b/stdlib/2and3/builtins.pyi
index a79300956..7a7245396 100644
--- a/stdlib/2and3/builtins.pyi
+++ b/stdlib/2and3/builtins.pyi
@@ -56,6 +56,7 @@ from typing import (
     Union,
     ValuesView,
     overload,
+    runtime_checkable,
 )
 from typing_extensions import Literal
 
@@ -1211,7 +1212,7 @@ if sys.version_info < (3,):
 # but we define it here as _PathLike to avoid import cycle issues.
 # See https://github.com/python/typeshed/pull/991#issuecomment-288160993
 _AnyStr_co = TypeVar("_AnyStr_co", str, bytes, covariant=True)
-
+@runtime_checkable
 class _PathLike(Protocol[_AnyStr_co]):
     def __fspath__(self) -> _AnyStr_co: ...