diff --git a/stdlib/mimetypes.pyi b/stdlib/mimetypes.pyi
index 128a05fa5..532cc5e3c 100644
--- a/stdlib/mimetypes.pyi
+++ b/stdlib/mimetypes.pyi
@@ -53,5 +53,4 @@ class MimeTypes:
     def guess_all_extensions(self, type: str, strict: bool = True) -> list[str]: ...
     def read(self, filename: str, strict: bool = True) -> None: ...
     def readfp(self, fp: IO[str], strict: bool = True) -> None: ...
-    if sys.platform == "win32":
-        def read_windows_registry(self, strict: bool = True) -> None: ...
+    def read_windows_registry(self, strict: bool = True) -> None: ...
diff --git a/tests/stubtest_allowlists/darwin.txt b/tests/stubtest_allowlists/darwin.txt
index d3f64a642..b7a297117 100644
--- a/tests/stubtest_allowlists/darwin.txt
+++ b/tests/stubtest_allowlists/darwin.txt
@@ -29,8 +29,6 @@ select.POLLMSG   # system dependent
 (termios.[A-Z0-9_]+)?
 
 # Exists at runtime, but missing from stubs
-mimetypes.MimeTypes.read_windows_registry
-
 _ctypes.dlclose
 _ctypes.dlopen
 _ctypes.dlsym
diff --git a/tests/stubtest_allowlists/linux.txt b/tests/stubtest_allowlists/linux.txt
index 930d4ed0f..316d9265f 100644
--- a/tests/stubtest_allowlists/linux.txt
+++ b/tests/stubtest_allowlists/linux.txt
@@ -14,7 +14,6 @@ signal.SIGINFO
 socket.[A-Z0-9_]+
 
 # Exists at runtime, but missing from stubs
-mimetypes.MimeTypes.read_windows_registry
 spwd.struct_spwd.sp_nam
 spwd.struct_spwd.sp_pwd